Video Analysis of Triops Heartbeat
•A stream of video is recorded
that documents heartbeat activity
before and while an experimental
solution is administered (~4 min)
•Video is enhanced to accentuate heart
–Two layers of high contrast filters
–Converted to grayscale and inverted
–Frame rate decreased to 15 FPS
–Sliced into 10 second clips
–Frames excised to eliminate partial beats
•Number of full beats counted
•Heartbeat rate calculated (BPM)
•Accurate to 2 BPM for a 5 second video recording